A4102
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 | Immunoblotting Mouse brain (Lane A, C) and rat brain (Lane B) cytosolic fractions were separated on SDS-PAGE, blotted with Rabbit Anti-ASAP1 (VG-17) (Cat. No. A4102) and developed with Goat Anti-Rabbit IgG Peroxidase (Cat. No. A0545) and chemiluminescent substrate. (Lanes A, B) Antibody dilution 1: 2,000; (Lane C) Antibody dilution 1: 2,000 + 20 μg/mL ASAP1 immunizing peptide. |
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A4102
Sigma
Anti-ASAP1/Centaurin β4 (VG-17) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| Synonym: | Anti-Arf GAP containing SH3, ANK repeat and PH/Centaurin β4 (VG-17) |
| MDL number: | MFCD04040185 |
Description
| Immunogen | synthetic peptide located near the C-terminus of mouse ASAP1 (amino acids 962-978) conjugated to KLH. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 130 kDa (doublet band) |
| species reactivity | mouse, human, rat |
| application(s) | immunoblotting: 1:2,000 using rat brain and mouse brain extracts |
| indirect immunofluorescence: 1:2,000 using mouse NIH3T3 fibroblast cells | |
| Gene Information | human ... ASAP1(50807) |
| rat ... Asap1(314961) | |
| mouse ... Asap1(13196) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
